diff --git a/docs/changes/newsfragments/388.bugfix b/docs/changes/newsfragments/388.bugfix
new file mode 100644
index 000000000..d50231d77
--- /dev/null
+++ b/docs/changes/newsfragments/388.bugfix
@@ -0,0 +1 @@
+Fix coordinates space transformation using ANTs by `Synchon Mandal`_
diff --git a/junifer/data/coordinates/_ants_coordinates_warper.py b/junifer/data/coordinates/_ants_coordinates_warper.py
index a6f204836..106da582e 100644
--- a/junifer/data/coordinates/_ants_coordinates_warper.py
+++ b/junifer/data/coordinates/_ants_coordinates_warper.py
@@ -55,19 +55,24 @@ class ANTsCoordinatesWarper:
 
         # Save existing coordinates to a tempfile
         pretransform_coordinates_path = (
-            element_tempdir / "pretransform_coordinates.txt"
+            element_tempdir / "pretransform_coordinates.csv"
         )
+        # Convert LPS to RAS
+        seeds[:, 0] *= -1
+        seeds[:, 1] *= -1
         np.savetxt(
             pretransform_coordinates_path,
             seeds,
             delimiter=",",
             # Add header while saving to make ANTs work
             header="x,y,z",
+            # Remove comment tag for header
+            comments="",
         )
 
         # Create a tempfile for transformed coordinates output
         transformed_coords_path = (
-            element_tempdir / "coordinates_transformed.txt"
+            element_tempdir / "coordinates_transformed.csv"
         )
         # Set antsApplyTransformsToPoints command
         apply_transforms_to_points_cmd = [
@@ -86,9 +91,14 @@ class ANTsCoordinatesWarper:
         )
 
         # Load coordinates
-        return np.loadtxt(
+        transformed_seeds = np.loadtxt(
             # Skip header when reading
             transformed_coords_path,
             delimiter=",",
             skiprows=1,
         )
+        # Convert RAS to LPS
+        transformed_seeds[:, 0] *= -1
+        transformed_seeds[:, 1] *= -1
+
+        return transformed_seeds
diff --git a/junifer/data/coordinates/_coordinates.py b/junifer/data/coordinates/_coordinates.py
index f75662a21..1b5a5e8a1 100644
--- a/junifer/data/coordinates/_coordinates.py
+++ b/junifer/data/coordinates/_coordinates.py
@@ -362,7 +362,7 @@ class CoordinatesRegistry(BasePipelineDataRegistry, metaclass=Singleton):
                 seeds = ANTsCoordinatesWarper().warp(
                     seeds=seeds,
                     target_data=target_data,
-                    warp_data=warper_spec,
+                    warp_data=inverse_warper_spec,
                 )
 
         return seeds, labels